منابع مشابه
Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization
Tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. In order to assess the presence and prevalence of various pathogens in southern Italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (RLB) hybridization. The study had been carried out in three re...
متن کاملDetection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization.
Application of reverse transcription (RT)-PCR to detect small round-structured viruses (SRSVs) from fecal specimens of patients with gastroenteritis has been insensitive because of the tremendous sequence heterogeneity between strains. We have designed two RT-PCR primer sets (G-1 and G-2) based on the nucleotide sequence diversity in the RNA polymerase gene of SRSVs belonging to two distinct ge...
متن کامل[Reverse hybridization and sequencing for genotyping the hepatitis C virus].
INTRODUCTION The methods for genotyping the hepatitis C virus have been much discussed. The aim of this study was to compare the methodologies of reverse hybridization and direct sequencing for genotyping the hepatitis C virus. METHODS Ninety-one plasma samples from patients attended at the Botucatu Medical School, São Paulo State University, were used. Genotyping by reverse hybridization was...
متن کاملSequencing by Hybridization by Cooperating Direct and Reverse Spectra
DNA sequencing by hybridization, potentially a powerful alternative to standard wet lab techniques, has received renewed interest after a novel probing scheme has been recently proposed whose performance for the first time asymptotically meets the information theory bound. After settlement of the question of asymptotic performance, there remains the issue of algorithmic fine tunings aimed at im...
متن کاملMultiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.24.9943